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HelpOrder Code BRCMG Brucella Antibody Screen, IgM and IgG, ELISA, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluating patients with suspected brucellosis
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BRCM | Brucella Ab Screen, IgM ELISA, S | No | Yes |
| BRCG | Brucella Ab Screen, IgG ELISA, S | No | Yes |
| BRCI | Brucella Ab Screen Interpretation | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BRUTA | Brucella Ab, Agglutination, S | Yes | No |
Testing Algorithm
If the Brucella antibody screen, IgM or IgG, result is either positive or equivocal, then confirmation by Brucella total antibody agglutination testing will be performed at an additional charge.
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Brucella Ab Screen, IgM/IgG ELISA, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
IgG SCREEN
Negative
IgM SCREEN
Negative
Reference values apply to all ages.
Method Description
Serum is tested using an enzyme-linked immunosorbent assay (ELISA) Test Kit containing microtiter strips with wells coated with Brucella abortus antigens (strain W99). In the first reaction step, diluted patient samples are incubated in the wells. Specific IgG or IgM antibodies, if present in the serum, will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labeled antihuman IgG or antihuman IgM (enzyme conjugate). After addition of the substrate, tetramethylbenzidine/hydrogen peroxide and a sulphuric acid stop solution, the resulting color reaction is measured photometrically at a wavelength of 450nm.(Package insert: Anti-Brucella abortus ELISA Test Instructions, Euroimmun Medizinische Labordiagnostika; IgM: 01//2019, IgG: 08/2020)
Day(s) Performed
Tuesday, Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86622 x 2-Brucella antibody, IgG and IgM
86622-Brucella total antibody, agglutination (if appropriate)
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.