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HelpOrder Code BTBAS Butabarbital, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions:
1. Draw blood immediately before the next scheduled dose.
2. Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Useful For
Monitoring of butabarbital therapy
Method Name
Gas Chromatography Mass Spectrometry (GC-MS)
Reporting Name
Butabarbital, SSpecimen Type
Serum RedSpecimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Therapeutic range:
2.0-3.0 mcg/mL Hypnosis
25 mcg/mL Sedation/Sleep
>30 mcg/mL Coma
Toxic concentration: >50 mcg/mL
Cutoff concentrations by gas chromatography mass spectrometry:
Butabarbital: 0.5 ng/mL
Method Description
Barbiturates are extracted from serum using solid-phase extraction techniques. The serum is buffered and eluted with organic solvent. The organic phase is dried, reconstituted, and the analysis performed by gas chromatography mass spectrometry using selected ion monitoring. The assay utilizes deuterated barbiturates as internal standards.(Unpublished Mayo method)
Day(s) Performed
Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80299