Home
HelpOrder Code CEE49 CD49d Cell Expression Evaluation, Varies
Ordering Guidance
This test should not be used as a shortened diagnostic panel. For a complete diagnostic B-cell, T-cell, or acute immunophenotyping panel, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies.
This test evaluates CD49d expression only. For CD20 expression, order CEE20 / CD20 Cell Expression Evaluation, Varies. For CD52 expression, order CEE52 / CD52 Cell Expression Evaluation, Varies.
Shipping Instructions
Specimen must arrive within 4 days of collection.
Necessary Information
The following information is required:
1. The therapeutic monoclonal antibody being used or considered
2. The pertinent hematologic diseases that have been diagnosed or considered
3. Specimen source
4. Date and time of collection
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole Blood
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or Green top (sodium heparin)
Specimen Volume: 10 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot
2. Label specimen as blood.
Specimen Stability Information: Ambient ≤ 4 days/Refrigerated ≤4 days
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or Green top (sodium heparin)
Specimen Volume: 1-5 mL
Collection Instructions:
1. Submission of bilateral specimens is not required.
2. Label specimen as bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Specimen Stability Information: Ambient/Refrigerated ≤4 days
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Detecting cell-surface antigens on malignant cells that are potential therapeutic antibody targets, specifically CD49d
Determining the eligibility of patients for monoclonal antibody therapies
Monitoring response to the therapeutic antibody
Testing Algorithm
A complete diagnostic B-cell, T-cell, or acute immunophenotyping panel is not performed. In some cases, a limited morphologic evaluation will be performed.
Method Name
Immunophenotyping
Reporting Name
CD49d Cell Expression Evaluation, VSpecimen Type
VariesSpecimen Minimum Volume
Blood: 3 mL
Bone Marrow Aspirate: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 4 days |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
Normal individuals have B lymphocytes, T lymphocytes, or myeloid cells that express the corresponding cell-surface antigens in question.
Method Description
Flow cytometric immunophenotyping of peripheral blood, bone marrow, or tissue-derived lymphocytes is performed to assess the expression of the cell-surface antigen corresponding to the monoclonal antibody therapeutic target. The following antibody panels will be used:
-Anti-CD49d assessment: CD19/CD49d/CD3/CD45
(Keren P, McCoy Jr JP, Carey J, eds. Flow Cytometry in Clinical Diagnosis. 4th ed. ASCP Press; 2007)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker
88185 x 3-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker
88187-Flow Cytometry Interpretation, 2 to 8 Markers