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Order Code CHIKG Chikungunya IgG, Antibody, Serum

Method Name

Only orderable as part of a profile. For more information see CHIKV / Chikungunya IgM and IgG, Antibody, Serum.

 

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Chikungunya IgG, Ab, S

Specimen Type

Serum


Specimen Required


Only orderable as part of a profile. For more information see CHIKV / Chikungunya IgM and IgG, Antibody, Serum.

 

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reference Values

Only orderable as part of a profile. For more information see CHIKV / Chikungunya IgM and IgG, Antibody, Serum.

 

Negative

Reference values apply to all ages.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86790

Day(s) Performed

Bimonthly on the second and forth Wednesday

Useful For

Aiding in the diagnosis of recent infection with Chikungunya virus detecting IgG antibodies in patients with recent travel to endemic areas and a compatible clinical syndrome

Specimen Minimum Volume

0.4 mL

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Description

For the Chikungunya virus IgG assay, polystyrene microwells are coated with recombinant Chikungunya antigen. Diluted serum samples and controls are incubated in the wells to allow anti-Chikungunya antibodies (if present in the sample) to react with the antigen. Nonspecific reactants are removed by washing. Next, peroxidase-conjugated antihuman antibody is added to the wells and will react with human antibodies bound to the antigen. Excess conjugate is removed by washing. Enzyme substrate and chromogen are added, and the color is allowed to develop. After adding the Stop Reagent, the resultant color change is quantified by a spectrophotometric reading of optical density (OD). Sample OD readings are compared with reference cut-off OD readings to determine the qualitative results.(Package inserts: Anti-Chikungunya virus ELISA IgG. , Euroimmun Ag; v. 12/20/2018)