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Order Code CIFA Cutaneous Immunofluorescence Antibodies, IgA, Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Confirming the presence of IgA antibodies to diagnose pemphigoid, pemphigus, epidermolysis bullosa acquisita, or bullous lupus erythematosus

Method Name

Indirect Immunofluorescence Assay (IFA)

Reporting Name

Cutaneous Immfluor. Ab (IgA), S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Reference Values

Report includes presence and titer of circulating antibodies. If serum contains basement membrane zone antibodies on split-skin substrate, patterns will be reported as:

1) Epidermal pattern, consistent with pemphigoid

2) Dermal pattern, consistent with epidermolysis bullosa acquisita

 

Negative in normal individuals

Method Description

Frozen sections of primate esophagus and sodium chloride-split primate skin are overlaid with dilutions of patient's serum, incubated, covered with fluorescein-conjugated IgA antiserum, and interpreted with a fluorescence microscope.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

88346

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CIFA Cutaneous Immfluor. Ab (IgA), S 104828-9

 

Result ID Test Result Name Result LOINC Value
610628 Cell Surface Ab IgA 104829-7
610629 Basement Membrane IgA 104830-5
610630 Primate Esophagus IgA 104833-9
610631 Primate Split Skin IgA 104834-7
610632 Other 48767-8