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Order Code CIFST Cutaneous Immunofluorescence Antibodies Titer, IgG, Serum


Specimen Required


Only orderable as a reflex. For more information see CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum.

 

Collection Container/Tube:

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Confirmation of positive IgG anti-cell surface (CS) and anti-basement membrane zone (BMZ) antibodies.

Method Name

Only orderable as a reflex. For more information see CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum.

 

Indirect Immunofluorescence Assay (IFA)

Reporting Name

CIFS Titer, IgG, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Only orderable as a reflex. For more information see CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum.

 

Negative in normal individuals

Method Description

Frozen sections of primate esophagus are overlaid with dilutions of patient's serum, incubated, covered with fluorescein-conjugated IgG antiserum, and interpreted with a fluorescence microscope.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

88350