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Order Code CIMT Coccidioides immitis/posadasii, Molecular Detection, PCR, Paraffin, Tissue

Useful For

Rapid detection of Coccidioides DNA

 

Aiding in the diagnosis of coccidioidomycosis using paraffin-embedded tissue specimens

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Coccidioides PCR, FFPE

Specimen Type

Tissue, Paraffin


Necessary Information


Specimen source is required.



Specimen Required


Preferred Paraffin-embedded tissue block:

Supplies: Tissue Block Container (T553)

Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)

Sources: Body tissue

Container/Tube: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.

 

Acceptable Paraffin-embedded tissue block:

Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)

Sources: Body tissue

Container/Tube: Sterile container for each individual cut section (scroll).

Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.


Specimen Minimum Volume

See Specimen Required.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue, Paraffin Ambient (preferred)
  Refrigerated 

Reject Due To

Any non-FFPE tissue blocks FFPE bone marrow FFPE slides FFPE body fluids Reject

Reference Values

Not applicable

Method Description

Following specimen processing, nucleic acids are extracted, and the extract transferred to individual self-contained cuvettes for amplification using the LightCycler real-time polymerase chain reaction (PCR) platform (Roche Applied Sciences). The LightCycler is an automated instrument that amplifies and monitors the development of target nucleic acid (amplicon) after each cycle of PCR. The detection of amplicon is based on fluorescence resonance energy transfer, which utilizes hybridization probes. The presence of the specific organism nucleic acid is confirmed by performing a melting curve analysis of the amplicon.(Binnicker MJ, Buckwalter SP, Eisberner JJ, et al: Detection of Coccidioides species in clinical specimens by real-time PCR. J Clin Microbiol 2007;45:173-178)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798