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Order Code CLFA Cryptococcus Antigen Screen with Titer, Spinal Fluid

Useful For

Aiding in the diagnosis of cryptococcosis

 

This test should not be performed as a screening procedure for the general population.

 

This test should not be used as a test of cure or to guide treatment decisions.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
CLFAT Cryptococcus Ag Titer, LFA, CSF Yes No

Testing Algorithm

If result is positive, Cryptococcus titer will be performed at an additional charge.

 

For more information see Meningitis/Encephalitis Panel Algorithm

Reporting Name

Cryptococcus Ag Screen w/Titer, CSF

Specimen Type

CSF


Specimen Required


Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Submit specimen from collection vial 2 (preferred), 3, or 4.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
CSF Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject

Reference Values

Negative

Reference values apply to all ages.

Method Description

The Cryptococcus antigen (CrAg) lateral flow assay is a sandwich immunochromatographic assay. Specimens and diluent are added to a test tube and the lateral flow device is added. The test uses specimen wicking to capture gold-conjugated, anti-cryptococcal antigen monoclonal antibodies and gold-conjugated control antibodies deposited on the test membrane. If cryptococcal antigen is present in the specimen, it binds to the gold-conjugated, anti-cryptococcal antigen antibodies. This complex wicks up the membrane and interacts with the test line, which has immobilized anti-cryptococcal antigen monoclonal antibodies. The antigen-antibody complex forms a sandwich at the test line causing a visible line to form. A valid test shows a visible line at the control line. Positive test results create 2 lines (control and specimen), while negative results form only the control line.(Package insert: CrAg Lateral Flow Assay. IMMY; Rev 06/27/2019)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87899-Cryptococcus screen

87899-Cryptococcus titer (if appropriate)

Method Name

Lateral Flow Assay (LFA)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.