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Order Code COXIS Coccidioides Antibody Screen with Reflex, Serum

Useful For

Detecting antibodies to Coccidioides immitis/posadasii

 

This assay should not be used for monitoring response to therapy.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RSCOC Coccidioides Ab, CompF/ImmDiff,S Yes, (order SCOC) No

Testing Algorithm

If result is reactive, then Coccidioides by complement fixation and immunodiffusion will be performed at an additional charge.

 

For more information see Meningitis/Encephalitis Panel Algorithm.

Reporting Name

Coccidioides Ab Screen w/Reflex, S

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

1.7 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Negative

 

Reference value applies to all ages

Method Description

Microwells are coated with recombinant Coccidioides complement fixing (CF) and tube precipitin (TP) antigens. Diluted serum specimens and controls are incubated in the wells, and if present, antibodies to TP and CF will bind to the adhered antigen. Nonspecific reactants are removed by washing; peroxidase-conjugated, secondary antihuman antibody is then applied to the wells and incubated. The conjugated secondary antibody will bind to the patient antibodies. Substrate solution is added to the wells, activating the peroxidase conjugate to develop a color reaction. Stop solution is added and the color change is quantified by measuring the optical density.(Package insert: clarus Cocci AB EIA. Immy; Revision 03/06/2020)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86635

86635 x3 (if appropriate)

Method Name

COXIS: Enzyme Immunoassay (EIA)

RSCOC: Complement Fixation (CF)/Immunodiffusion (ID)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.