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Order Code CSPCF Plasma Cell Proliferative Disorder, Pre-Analysis Cell Sorting, Bone Marrow


Specimen Required


Only orderable as a reflex. See PCPDS / Plasma Cell Proliferative Disorder, High Risk with Reflex Probes, Diagnostic FISH Evaluation, Bone Marrow

 

Specimen Type: Bone marrow

Preferred: Yellow top (ACD solution A or B)

Acceptable: Lavender top (EDTA) or green top (heparin)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix bone marrow

2. Send bone marrow specimen in original tube. Do not aliquot.


Useful For

Aiding in the diagnosis of new cases of multiple myeloma or other plasma cell proliferative disorders

 

Sorting plasma cells for fluorescence in situ hybridization analysis

Method Name

Only orderable as a reflex. See PCPDS / Plasma Cell Proliferative Disorder, High Risk with Reflex Probes, Diagnostic FISH Evaluation, Bone Marrow

 

Flow Cytometric Cell Selection

Reporting Name

PCPDS Pre-Analysis Cell Sorting, BM

Specimen Type

Bone Marrow

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Bone Marrow Ambient (preferred) 4 days
  Refrigerated  4 days

Reject Due To

Gross hemolysis Reject
Other Fully clotted

Reference Values

Only orderable as a reflex. See PCPDS / Plasma Cell Proliferative Disorder, High Risk with Reflex Probes, Diagnostic FISH Evaluation, Bone Marrow

 

An interpretive report will be provided.

Method Description

Selection of plasma cells using fluorescence activated cell sorting is the most direct and robust method of obtaining relatively pure plasma cell populations for fluorescence in situ hybridization assessment.(Instruction manual: BD FACSMelody Cell Sorter User's Guide. Revision 3. BD Biosciences; 03/2020)

Day(s) Performed

Specimens processed: Monday through Sunday

Results reported: Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

88184-Flow Cytometry; first cell surface, cytoplasmic or nuclear marker

88185 x 5-Flow Cytometry, additional cell surface, cytoplasmic or nuclear marker (each)