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HelpOrder Code CYSTG Cysticercosis (Taenia solium), IgG, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
Aid for the diagnosis of cysticercosis caused by infection with Taenia solium
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Cysticercosis (T. solium), IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a protein A - alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of p-nitrophenyl phosphate (pNPP) substrate, which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of T solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.(Package insert: Taenia solium Enzyme immunoassay for the diagnosis of human cysticercosis. Bordier Affinity Products SA; 04/2018)
Day(s) Performed
Tuesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682