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HelpOrder Code CZPS Clonazepam and 7-Aminoclonazepam, Serum
Test Down Notes
Effective December 14, 2023: This test is temporarily unavailable due to analytic issues. The downtime is expected to be >30 days. Order FCLMS as an alternate. See test notification here.
Useful For
Assessing patient compliance
Monitoring for appropriate therapeutic level
Assessing clonazepam toxicity
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Clonazepam and 7-Aminoclonazepam, SSpecimen Type
Serum RedSpecimen Required
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.2 mL
Collection Instructions:
1. Draw blood immediately before next scheduled dose (minimum 12 hours after last dose).
2. Within 2 hours of collection, the specimen must be centrifuged and the serum aliquoted into a plastic vial.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 14 days | |
| Frozen | 28 days | ||
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | Reject |
Reference Values
Clonazepam
Anticonvulsant: 20-70 ng/mL
Anxiolytic: 4-80 ng/mL
Some individuals may show therapeutic response outside of these ranges or may display toxicity within the therapeutic range, thus interpretation should include clinical evaluation.
Note: Therapeutic ranges are for specimens collected at trough (ie, immediately before next scheduled dose). Levels may be elevated in non-trough specimens.
Method Description
Clonazepam and 7-aminoclonazepam are extracted from serum using a liquid-liquid extraction technique. The organic layer is removed, dried, and reconstituted. Analysis is by liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Monday, Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80299
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.