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HelpOrder Code DENGM Dengue Virus Antibody, IgG and IgM, Serum
Useful For
Aiding in the diagnosis of dengue virus infection
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DENG | Dengue Virus Ab, IgG, S | No | Yes |
| DENM | Dengue Virus Ab, IgM, S | No | Yes |
| DNABI | Dengue Ab Interpretation | No | Yes |
Reporting Name
Dengue Virus Ab, IgG and IgM, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
Dengue virus antibody, IgG: Negative
Dengue virus antibody, IgM: Negative
Reference values apply to all ages.
Method Description
Dengue virus IgM:
In this enzyme-linked immunosorbent assay (ELISA), samples and controls are diluted in sample dilution buffer and incubated in microtiter wells coated with antihuman IgM antibodies. This incubation is followed by incubation with dengue-derived recombinant antigens (DENRA) and normal cell antigen separately. After incubation and washing, the wells are treated with a DEN-specific monoclonal antibody labeled with horseradish peroxidase (HRP). After a second incubation and washing step, the wells are incubated with tetramethylbenzidine (TMB) substrate. Acid stop is added, and absorbance at 450 nm is read. The ratio of absorbencies of the DENRA and the control antigen wells determines whether the result is positive or negative.(Package insert: InBiOS DENV Detect IgM CAPTURE ELISA. InBios International, Inc; Revision 10/2019)
Dengue virus IgG:
In this enzyme-linked immunosorbent assay, controls and diluted samples are incubated in microtiter wells coated with monoclonal antibody bound to dengue-derived recombinant antigens (DENRA). After incubation and washing, wells are treated with IgG antibody labeled with horseradish peroxidase. After a second incubation and washing, wells are incubated with tetramethylbenzidine substrate. Acid stop is added, and absorbance at 450 nm is measured. The ratio of the absorbencies of the DENRA and the control wells determines whether a result is positive or negative.(Package insert: InBiOS DENV Detect IgG ELISA. InBios International, Inc; Revision 05/01/2018)
Day(s) Performed
Tuesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
IgM-86790
IgG-86790
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.