Home
HelpOrder Code DHES1 Dehydroepiandrosterone Sulfate, Serum
Additional Codes
MAYO CODE: DHES1
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Diagnosis and differential diagnosis of hyperandrogenism (in conjunction with measurements of other sex steroids)
An adjunct in the diagnosis of congenital adrenal hyperplasia
Diagnosis and differential diagnosis of premature adrenarche
Method Name
Immunoenzymatic Assay
Reporting Name
Dehydroepiandrosterone Sulfate, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
MALES
1-14 days: DHEA-S levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.
Tanner Stages*Â
|
Mean |
Age |
Reference Range (mcg/dL) |
|
Stage I |
>14 days |
11-120 |
|
Stage II |
11.5 years |
14-323 |
|
Stage III |
13.6 years |
5.5-312 |
|
Stage IV |
15.1 years |
29-412 |
|
Stage V |
18.0 years |
104-468 |
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (±) 2 years. For boys, there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) is usually reached by age 18.
18-30 years: 105-728 mcg/dL
31-40 years: 57-522 mcg/dL
41-50 years: 34-395 mcg/dL
51-60 years: 20-299mcg/dL
61-70 years: 12-227 mcg/dL
≥71 years: 6.6-162 mcg/dL
FEMALES
1-14 days: DHEA-S levels in newborns are very elevated at birth but fall to prepubertal levels within a few days.
Tanner Stages*Â
|
Mean |
Age |
Reference Range (mcg/dL) |
|
Stage I |
>14 days |
16-96 |
|
Stage II |
10.5 years |
22-184 |
|
Stage III |
11.6 years |
11-296 |
|
Stage IV |
12.3 years |
17-343 |
|
Stage V |
14.5 years |
57-395 |
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (±) 2 years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) is usually reached by age 18.
18-30 years: 83-377 mcg/dL
31-40 years: 45-295 mcg/dL
41-50 years: 27-240 mcg/dL
51-60 years: 16-195 mcg/dL
61-70 years: 9.7-159
≥71 years: 5.3-124 mcg/dL
Method Description
The Access DHEA-S assay is a competitive binding immunoenzymatic assay. A sample is added to a reaction vessel with paramagnetic particles coated with goat anti-rabbit:rabbit anti-DHEA-S and DHEA-S alkaline phosphatase conjugate in TRIS-buffered protein solution. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is inversely proportional to the concentration of DHEA-S in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve.(Package insert: Access DHEA-S. Beckman Coulter Inc; 04/2023)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82627
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.