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Order Code DIS Human Leukocyte Antigens (HLA) Class I and II Disease Association Typing, Low Resolution, Blood


Ordering Guidance


This test should be ordered if both human leukocyte antigen (HLA) Class I and Class II results are wanted for disease association.

 

If only needing patient's HLA Class I typing for disease association, order 1DIS / Human Leukocyte Antigens (HLA)-A-B-C Disease Association Typing Low Resolution, Blood.

 

If only needing patient's HLA Class II typing for disease association, order 2DIS / Human Leukocyte Antigens (HLA)-DR-DQ Disease Association Typing Low Resolution, Blood.



Specimen Required


Container/Tube: Yellow top (ACD solution A or B)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood in original tube. Do not aliquot.

Additional Information: Specimen acceptability is based on extracted DNA concentration and not sample age.


Useful For

Identifying class I and II human leukocyte antigens (HLA) for potential disease associations or markers for drug hypersensitivity

Profile Information

Test ID Reporting Name Available Separately Always Performed
1DIS HLA-A-B-C DisAssoc Typing LowRes,B Yes Yes
2DIS HLA-DR-DQ DisAssoc Typing LowRes,B Yes Yes

Method Name

Polymerase Chain Reaction (PCR)/Next-Generation Sequencing (NGS)

Reporting Name

HLA CI-CII DisAssoc Typing LowRes,B

Specimen Type

Whole Blood ACD

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD Ambient (preferred)
  Refrigerated 

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Not applicable

Method Description

Next-generation sequencing is used to type for Class I alleles (A, B, and C) and Class II alleles (DRB1, DRB3/4/5, DQB1, DQA1, DPB1, and DPA1) from genomic DNA. This method uses strictly controlled polymerase chain reaction (PCR) conditions for DNA amplification. The PCR amplicons are processed and sequenced via the Illumina MiSeq instrument. The output files are analyzed in provided software, which compares the data against the IMGT/HLA database to assign the molecular typing.(Package inserts: Holotype HLA Kit. Omixon; v3.0.1, 08/16/2019; NGSgo HLA Kit. GenDx; v2, 02/2021)

 

For resolution of an allelic ambiguity or in select cases, the following additional methodologies may be utilized:

-Sequence-based typing (SBT) by Sanger sequencing(Package insert: SeCore Sequencing and GSSP Kits. One Lambda, Inc; Rev 3, 02/06/2021)

-SBT by sequence-specific primers (SSP)(Package insert: Olerup SSP HLA typing kits including Taq Polymerase. CareDx; Rev 04, 12/2020)

-Reverse sequence-specific oligonucleotides (SSO)(Package insert: LABType SSO Typing Test. One Lambda, Inc.; Rev 04, 11/11/2019)

Day(s) Performed

Monday, Thursday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81370

81376 x3