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HelpOrder Code DIS Human Leukocyte Antigens (HLA) Class I and II Disease Association Typing, Low Resolution, Blood
Ordering Guidance
This test should be ordered if both human leukocyte antigen (HLA) class I and class II results are wanted for disease association.
If only needing patient's HLA class I typing for disease association, order 1DIS / Human Leukocyte Antigens (HLA)-A-B-C Disease Association Typing Low Resolution, Blood.
If only needing patient's HLA class II typing for disease association, order 2DIS / Human Leukocyte Antigens (HLA)-DR-DQ Disease Association Typing Low Resolution, Blood.
Specimen Required
Container/Tube: Yellow top (ACD solution A or B)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood in original tube. Do not aliquot.
Additional Information: Specimen acceptability is based on extracted DNA concentration and not sample age.
Useful For
Identifying class I and II human leukocyte antigens (HLA) for potential disease associations or markers for drug hypersensitivity
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| 1DIS | HLA-A-B-C DisAssoc Typing LowRes,B | Yes | Yes |
| 2DIS | HLA-DR-DQ DisAssoc Typing LowRes,B | Yes | Yes |
Method Name
Polymerase Chain Reaction (PCR)/Next-Generation Sequencing (NGS)
Reporting Name
HLA CI-CII DisAssoc Typing LowRes,BSpecimen Type
Whole Blood ACDSpecimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Extracted DNA | Reject |
Reference Values
Not applicable
Method Description
Next-generation sequencing is used to type for class I alleles (A, B, and C) and class II alleles (DRB1, DRB3/4/5, DQB1, DQA1, DPB1, and DPA1) from genomic DNA. This method uses strictly controlled polymerase chain reaction (PCR) conditions for DNA amplification. The PCR amplicons are processed and sequenced via the Illumina MiSeq instrument. The output files are analyzed in provided software, which compares the data against the IMGT/HLA database to assign the molecular typing.(Package insert: NGSgo HLA Kit. GenDx; v3, 06/2022)
For resolution of an allelic ambiguity or in select cases, the following additional methodologies may be utilized:
-Sequence-based typing (SBT) by Sanger sequencing(Package insert: SeCore Sequencing and GSSP Kits. One Lambda, Inc; Rev 3, 02/06/2021)
-Reverse sequence-specific oligonucleotides (SSO)(Package insert: LABType SSO Typing Test. One Lambda, Inc.; Rev 04, 11/11/2019)
Day(s) Performed
Monday, Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81370
81376 x3