Order Code DOGPF Dog Dander, IgE, with Reflex to Dog Dander Components, IgE, Serum
Ordering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluating patients with suspected dog dander allergy
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
DOGPR | Dog Dander Components, IgE, S | No | No |
Testing Algorithm
Testing begins with analysis of dog dander IgE. If dog dander IgE is negative (<0.10 kU/L), testing is complete.
If dog dander IgE is 0.10 kU/L or more, then 6 dog dander components (Can f 1, Can f 2, Can f 3, Can f 4, Can f 5, Can f 6) are performed at an additional charge.
Special Instructions
Method Name
Fluorescent Enzyme Immunoassay (FEIA)
Reporting Name
Dog Dander Component Reflex, SSpecimen Type
SerumSpecimen Minimum Volume
0.7 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 90 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
Class |
IgE kU/L |
Interpretation |
0 |
<0.10 |
Negative |
0/1 |
0.10-0.34 |
Borderline/equivocal |
1 |
0.35-0.69 |
Equivocal |
2 |
0.70-3.49 |
Positive |
3 |
3.50-17.4 |
Positive |
4 |
17.5-49.9 |
Strongly positive |
5 |
50.0-99.9 |
Strongly positive |
6 |
≥100 |
Strongly positive |
Concentrations of 0.70 kU/L or more (class 2 and above) will flag as abnormally high.
Reference values apply to all ages.
Method Description
Specific IgE from the patient's serum reacts with the allergen of interest, covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away, and the bound complex incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Phadia AB; Rev 06/2020)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
86003
Forms
If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.