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HelpOrder Code DOXA1 Oxalate Analysis, Hemodialysate
Specimen Required
Specimen Type: Dialysate fluid
Patient Preparation: Patient should avoid taking vitamin C supplements for 24 hours prior to dialysis
Supplies: Urine Tubes, 10 mL (T068)
Container/Tube: Plastic, 10-mL urine tube or a clean, plastic aliquot container with no metal cap or glued insert
Specimen Volume: 5 mL
Collection Instructions: Adjust the pH of the specimen to 2.5 to 3.0 with 6M Hydrochloric Acid
Additional Information: Nonacidified frozen hemodialysate delivered to the laboratory within 3 days from collection will be accepted and the following comment will be added to the result: In nonacidified hemodialysate stored frozen, oxalate values may increase spontaneously.
Useful For
Determining the amount of oxalate removed during a dialysis session
Individualizing the dialysis prescription of hyperoxaluric patients
Method Name
Enzymatic
Reporting Name
Oxalate, Dialysate FluidSpecimen Type
Dialysate FluidSpecimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Dialysate Fluid | Frozen | 14 days |
Reject Due To
| All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Reference Values
Not applicable
Method Description
This is an enzymatic method based on the reduction of oxalate by oxalate oxidase. The reaction releases hydrogen peroxide, which in the presence of peroxidase reacts with a dye to give a colored end point that is measured using a BioTek EPOCH plate spectrophotometer at 590 nm.(Package insert: Trinity Biotech, Oxalate Kit, Jamestown, NY, V. 07/2016)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83945
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.