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HelpOrder Code DSAC Disaccharidase Activity Panel, Tissue
Shipping Instructions
Freeze immediately after collection and ship frozen.
Specimen Required
Source: Intestinal biopsy
Supplies: 15 mL Tissue Tube-DSAC (T993)
Container/Tube: 15 mL tissue tube
Specimen Volume: 5 mg
Collection Instructions:
1. Place specimen in tissue tube.
2. Specimen should not be placed on gauze, filter paper, or swabs/wooden sticks and should not have any saline, water, support, or embedding material added.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Useful For
Evaluation of patients who present with signs or symptoms suggestive of disaccharidase disorders
This test is not intended for carrier detection.
Genetics Test Information
Diagnostic testing for patients with clinical signs and symptoms suspicious of a disaccharidase deficiency.
Method Name
Spectrophotometry
Reporting Name
Disaccharidase Activity Panel, TsSpecimen Type
TissueSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue | Frozen | 28 days | OTHER |
Reject Due To
| Specimens sent in formaldehyde, saline, or any supportive media Specimens placed on gauze, filter paper, foil, or swabs/wooden sticks |
Reject |
Reference Values
Lactase: ≥14.0 nmol/min/mg protein
Sucrase: ≥19.0 nmol/min/mg protein
Maltase: ≥70.0 nmol/min/mg protein
Palatinase: ≥6.0 nmol/min/mg protein
Glucoamylase: ≥8.0 nmol/min/mg protein
Method Description
Disaccharidase activities are measured by incubating intestinal homogenates with an appropriate disaccharide substrate, which releases glucose. Glucose production is determined by a glucose oxidase reagent mixture that oxidizes glucose to produce peroxide, which reacts with phenol aminophenazone to form a red quinoneimine dye. The concentration of quinoneimine is determined spectrophotometrically, and the concentration of liberated glucose is calculated from a glucose standard curve. The amount of glucose produced is proportional to the disaccharidase activity in the intestinal homogenate. Disaccharidase activities are expressed as nanomoles of glucose per minute per milligram of protein (nmol/min/mg prot).(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82657