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Order Code EDN Eosinophil Derived Neurotoxin, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial within 12 hours of collection. Serum cannot sit on either gel or cells for longer than 12 hours.


Useful For

Evaluating patients suspected to have a condition associated with eosinophilia or hypereosinophilia

 

Evaluating patients with elevated peripheral blood eosinophil counts

 

Managing patients with elevated eosinophil-derived neurotoxin in the context of eosinophil-associated diseases

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

Reporting Name

Eosinophil Derived Neurotoxin, S

Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  21 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK
Heat-treated specimen Reject
If serum is on cell pellet or gel for >12 hours Reject

Reference Values

<70 mcg/L: Normal

70-99 mcg/L: Borderline

≥100 mcg/L: Elevated

Reference values apply to all ages.

Method Description

Anti-eosinophil derived neurotoxin (EDN) antibodies, covalently bound to lmmunoCAP, bind to EDN in the patient sample. After washing, enzyme labeled antibodies against EDN are added to form a complex. Following incubation, the unbound enzyme labelled anti-EDN antibodies are washed away, and the bound complex is incubated with a developing agent. After the reaction has been stopped, the fluorescence of the eluate is measured. The response value correlates to the amount of EDN in the sample and a calibration curve is used to transform the response values to concentrations.(Package insert: Phadia ImmunoCAP EDN Assay Kit. Thermo Scientific; Rev 09/2020)

Day(s) Performed

Tuesday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83520