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Order Code EGWTP Egg White Component Profile, Serum


Ordering Guidance


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial 

Specimen Volume: 0.75 mL; if needed, 0.5 mL for every 5 allergens requested

Collection Instructions: Centrifuge and aliquot serum into a plastic vial. 


Useful For

Identifying egg white allergens:

-Responsible for allergic disease and/or anaphylactic episode

-To confirm sensitization prior to beginning immunotherapy

 

This test is not useful for patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or for patients in whom the medical management does not depend upon identification of allergen specificity.

Profile Information

Test ID Reporting Name Available Separately Always Performed
EGG Egg White, IgE Yes Yes
OVAL Ovalbumin, IgE Yes Yes
OVMU Ovomucoid, IgE Yes Yes

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

Reporting Name

Egg White Component Profile, S

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL
For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  90 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Class

IgE kU/L

Interpretation

0 

<0.10 

Negative 

0/1 

0.10-0.34 

Borderline/equivocal 

1 

0.35-0.69 

Equivocal 

2 

0.70-3.49 

Positive 

3 

3.50-17.4 

Positive 

4 

17.5-49.9 

Strongly positive 

5 

50.0-99.9 

Strongly positive 

6 

≥100 

Strongly positive 

 

Reference values apply to all ages.

Method Description

Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away, and the bound complex incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Phadia; Rev 06/2020)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86003 x 1

86008 x 2

Forms

If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.