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HelpOrder Code EHOLG Entamoeba histolytica Antibody, Serum
Ordering Guidance
Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OPE / Ova and Parasite, Travel History or Immunocompromised, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
As an adjunct in the diagnosis of extraintestinal, invasive amebiasis
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
E. histolytica Ab, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, a horseradish peroxidase-labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step, unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Entamoeba histolytica ELISA IgG Test kit. Gold Standard Diagnostics; GSD-ENTG-120705.F; 01/12/2021)
Day(s) Performed
Tuesday, Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86753
Forms
If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.