Sign in →

Order Code EHRC Ehrlichia chaffeensis (HME) Antibody, IgG, Serum

Additional Codes

Mayo Test ID
EHRC

Reporting Name

Ehrlichia Chaffeensis (HME) Ab, IgG

Useful For

An adjunct in the diagnosis of ehrlichiosis

 

Seroepidemiological surveys of the prevalence of the infection in certain populations

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-inactivated specimen Reject

Reference Values

<1:64

Reference values apply to all ages.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Friday

Method Name

Immunofluorescence Assay (IFA)

CPT Code Information

86666

Method Description

The patient's serum is diluted and is placed in microscopic slide wells that have been coated with Ehrlichia chaffeensis-infected cells. After incubation, the slides are washed and a fluorescein-isothiocyanate conjugate is added to each well. The slides are then read using a fluorescence microscope and significant fluorescent staining of intracellular organisms constitutes a positive reaction.(Dumler JS, Asanovich KM, Bakken JS, Richter P, Kimsey R, Madigan JE. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic Ehrlichia. J Clin Microbiol. 1995;33[5]:1098-1103; Pancholi P, Kolbert CP, Mitchell PD, et al. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis. 1995;172[4]:1007-1012; Dawson JE, Fishbein DB, Eng TR, Redus MA, Green NR. Diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity. J Infect Dis. 1990;162[1]:91-95; package insert: Ehrlichia chaffeensis IFA IgG. DiaSorin Molecular; 08/2016)

Testing Algorithm

For more information see Acute Tick-Borne Disease Testing Algorithm

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.