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HelpOrder Code ETGX Ethyl Glucuronide Confirmation, Chain of Custody, Random, Urine
Specimen Required
Supplies: Chain of Custody Kit (T282)
Container/Tube: Chain-of-custody kit containing the specimen containers, seals, and documentation is required.
Specimen Volume: 5 mL
Collection Instructions: Collect specimen in the provided container, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.
Additional Information: Submitting less than 5 mL will compromise our ability to perform all necessary testing.
Forms
1. Chain of Custody Request is included in the Chain-of-Custody Kit (T282).
2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.
Useful For
Monitoring abstinence in clinical and justice system settings using ethyl glucuronide and ethyl sulfate as direct biomarkers or metabolites of ethanol
This chain-of-custody test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was always under the control of personnel involved with testing the specimen; this control implies that the opportunity for specimen tampering would be limited.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COCH | Chain of Custody Processing | No | Yes |
| ADLTX | Adulterants Survey, CoC, U | Yes | Yes |
Testing Algorithm
Adulterants testing will be performed on all chain-of-custody urine samples as per regulatory requirements.
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Ethyl Glucuronide Conf, CoC, USpecimen Type
UrineSpecimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 72 hours |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
Negative
Cutoff concentrations: 500 ng/mL
Method Description
The assay uses an ethyl glucuronide recombinant antibody. It is based on the competition of ethyl glucuronide labeled enzyme glucose-6-phosphate dehydrogenase (G6PD) and the free drug in the urine sample for the fixed amount of antibody binding sites. In the absence of the free drug in the sample, the antibody binds the drug enzyme conjugate and enzyme activity is inhibited. This creates a dose response relationship between drug concentration in the urine and enzyme activity. The enzyme G6PD activity is determined at 340 nm spectrophotometrically by the conversion of nicotinamide adenine dinucleotide (NAD[+] to NADH.(Package insert: ETG. Immunalysis; 03/2019)
The urine sample is diluted with internal standard in 0.1% formic acid for detection by tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Monday, Wednesday, Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80307
80321
G0480 (if appropriate)