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HelpOrder Code FHBG Hepatitis B Virus Genotyping
Reporting Name
Hepatitis B Virus GenotypePerforming Laboratory
ARUP LaboratoriesSpecimen Type
Plasma EDTASpecimen Required
Draw blood in lavender (EDTA) tube(s). Spin down and send 2 mL plasma frozen in a plastic vial.
Required: 1. Viral Load
2. Viral Load Date
Note: Red-top serum and serum gel tube(s) are acceptable.
Note: This test may be unsuccessful if the HBV Viral load is less than log 3.0 or 1,000 IU/mL of plasma.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen (preferred) | 42 days | |
| Refrigerated | 7 days | ||
| Ambient | 72 hours |
Reference Values
Interpretive Information: Hepatitis B Virus Genotype
HBV genotype and resistance interpretation is provided by SeqHepB software from Evivar Medical. The following mutations are reported: reverse transcriptase L80I/V, Il69T, V173L, L180M, A181S/T/V, T184A/C/F/I/G/S/M/L, S202C/G/I, M204I/V, N236T, M250I/L/V; surface antigen P120T, D144A, G145R.
Both the HBV RT polymerase and the HBsAg encoding regions are sequenced. Resistance and surface antigen mutations are reported. In addition, the major HBV genotypes are identified. Mutations in viral sub-populations below 20% of total may not be detected.
This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc.
Day(s) Performed
Tuesday, Friday
CPT Code Information
87912
Reject Due To
| Hemolysis: | NA |
| Thawing: | Warm OK; Cold OK |
| Lipemia: | NA |
| Icterus: | NA |
| Other: | Heparinized specimens |
Method Name
Polymerase Chain Reaction/Sequencing