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HelpOrder Code GFAPP Glial Fibrillary Acidic Protein (GFAP), Plasma
Shipping Instructions
Send refrigerated.
Specimen Required
Patient Preparation:
Fasting: 8 hours, required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL plasma
Collection Information: Centrifuge and aliquot plasma into a plastic vial.
Useful For
As a biomarker of astrocyte activation related to brain injury and various neurological disorders
Method Name
Chemiluminescent Enzyme Immunoassay (CLEIA)
Reporting Name
Glial Fibrillary Acidic Protein, PSpecimen Type
EDTA PlasmaSpecimen Minimum Volume
Plasma: 0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| EDTA Plasma | Refrigerated (preferred) | 14 days |
| Frozen | 90 days | |
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Reference Values
<40 years: ≤32.6 pg/mL
40-49 years: ≤50.5 pg/mL
50-59 years: ≤67.5 pg/mL
60-69 years: ≤90.3 pg/mL
≥ 70 years: ≤120.8 pg/mL
Method Description
The Lumipulse G GFAP Immunoreaction is an assay system for the quantitative measurement of glial fibrillary acidic protein (GFAP) in plasma specimens based on chemiluminescent enzyme immunoassay technology by a specific two-step immunoassay method on the Lumipulse G System. The specimen and assay-specific diluent are added to the antibody-coated particle solution. The GFAP in the specimen specifically binds to anti-GFAP monoclonal mouse antibody on the particles and antigen-antibody immunocomplexes are formed. The particles are washed and rinsed to remove unbound materials. Alkaline phosphatase-labeled anti-GFAP monoclonal antibodies specifically bind to immunocomplexes on the particles. The particles are washed and rinsed to remove unbound materials. The substrate solution is added and mixed with the particles. 3-(2'-Spiroadamantyl)-4-methoxy-4-(3"-phosphoryloxy)-phenyl-1,2-dioxetane (AMPPD) contained in the substrate solution is dephosphorylated by the catalysis of alkaline phosphatase indirectly conjugated to particles. Luminescence (at a maximum wavelength of 477 nm) is generated by the cleavage reaction of dephosphorylated AMPPD. The luminescent signal reflects the amount of GFAP present in the sample.(Package insert: Lumipulse G GFAP. Fujirebio Inc; ver 1, 07/2024)
Day(s) Performed
Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| GFAPP | Glial Fibrillary Acidic Protein, P | 97604-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| GFAPP | Glial Fibrillary Acidic Protein, P | 97604-3 |