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Order Code GSH Glutathione, Blood


Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: Yellow top (ACD solution A)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Useful For

Evaluation of neonatal hyperbilirubinemia, favism or chronic or episodic hemolysis or jaundice

 

Evaluation for gamma-glutamylcysteine synthetase deficiency

 

Evaluation for glutathione synthetase deficiency causing hemolytic anemia

 

Evaluation for generalized glutathione synthetase deficiency with 5-oxoprolinuria

Method Name

Kinetic Spectrophotometry (KS)

Reporting Name

Glutathione, B

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject
Frozen Reject
Clotted (partial or whole) Reject

Reference Values

≥12 months: 46.9-90.1 mg/dL RBC

Reference values have not been established for patients younger than 12 months.

Method Description

Virtually all of the non-protein sulfhydryl of red cells is in the form of reduced glutathione (GSH). 5,5'-dithiobis (2-nitrobenzoic acid) is a disulfide compound, which is readily reduced by sulfhydryl compounds, forming a highly colored yellow anion. The absorbance of this resultant yellow substance is measured by 412 nm and compared to that of a known standard.(Beutler E. Red cell metabolism. In: A Manual of Biochemical Methods. 2nd ed. Grune and Stratton, 1984; Alisik M, Neselioglu S, Erel O. A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes, J Lab Med. 2019:43[5], 269-277. doi:10.1515/labmed-2019-0098)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82978

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GSH Glutathione, B 2383-8

 

Result ID Test Result Name Result LOINC Value
608409 Glutathione, B 2383-8

Day(s) Performed

Monday through Friday

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.