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Order Code GSH Glutathione, Blood


Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution B)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not transfer blood to other containers.


Useful For

Evaluation of neonatal hyperbilirubinemia, favism or chronic or episodic hemolysis or jaundice

 

Evaluation for gamma-glutamylcysteine synthetase deficiency

 

Evaluation for glutathione synthetase deficiency causing hemolytic anemia

 

Evaluation for generalized glutathione synthetase deficiency with 5-oxoprolinuria

Method Name

Kinetic Spectrophotometry (KS)

Reporting Name

Glutathione, B

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject

Reference Values

≥12 months: 46.9-90.1 mg/dL RBC

Reference values have not been established for patients who are younger12 months of age.

Method Description

Virtually all of the non-protein sulfhydryl of red cells is in the form of reduced glutathione (GSH). 5,5'-dithiobis (2-nitrobenzoic acid) is a disulfide compound, which is readily reduced by sulfhydryl compounds, forming a highly colored yellow anion. The absorbance of this resultant yellow substance is measured by 412 nm and compared to that of a known standard.(Beutler E: Red cell metabolism. In: A Manual of Biochemical Methods. 2nd ed. Grune and Stratton, 1984; Alisik M, Neselioglu S, Erel O. A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes, J Lab Med. 2019:43(5), 269-277. doi:10.1515/labmed-2019-0098)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82978

Day(s) Performed

Monday through Friday

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.