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Order Code HEVM Hepatitis E Virus IgM Antibody Screen with Reflex to Confirmation, Serum

Reporting Name

HEV IgM Ab Screen, S

Useful For

Diagnosis of acute or recent (<6 months) hepatitis E infection

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HEVML HEV IgM Ab Confirmation, S Yes No

Testing Algorithm

If hepatitis E virus (HEV) IgM antibody screen is reactive or borderline, HEV IgM antibody confirmation will be performed at an additional charge.

 

For more information see Hepatitis E: Testing Algorithm for Diagnosis and Management.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum SST


Necessary Information


Date of collection is required.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Serum SST Frozen (preferred)
  Refrigerated  24 hours

Reference Values

Negative

Day(s) Performed

Tuesday, Thursday

CPT Code Information

86790

Method Description

This is a qualitative, in vitro test for the detection and identification of IgM antibodies specifically against hepatitis E virus (HEV) in human serum. This assay is a screening test based on the principle of an indirect sandwich enzyme-linked immunosorbent assay.

 

Highly purified recombinant HEV-ORF2 viral antigens (specific for HEV genotypes 1 and 3) are fixed to microplate wells. Diluted patient serum specimens are incubated in the wells, in which antibodies bind specifically to the HEV recombinant antigens coating the surface of the wells. Unbound antibodies are then washed away. Anti-human immunoglobulin antibodies (IgM), which are coupled to horseradish peroxidase, are then added to the wells and incubated. Unbound conjugate antibodies are then washed away. Specifically, bound antibodies are detected by a peroxidase-catalyzed color reaction. Intensity of the color, as measured with a photometric analyzer, is proportionate to the quantity of bound HEV IgM antibodies present in the serum specimen.(Unpublished Mayo method)

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Enzyme Immunoassay (EIA)

Forms

If not ordering electronically, complete, print, and send 1 of the following:

-Gastroenterology and Hepatology Test Request (T728)

-Infectious Disease Serology Test Request (T916)

-Microbiology Test Request (T244)