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Order Code HEVQU Hepatitis E Virus RNA Detection and Quantification, Real-Time RT-PCR, Serum


Shipping Instructions


1. Freeze serum immediately, and ship specimen frozen on dry ice.

2. If shipment will be delayed for more 24 hours, freeze serum at -20 to -80° C (up to 35 days) until shipment on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1.8 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.


Useful For

Virologic detection and confirmation of hepatitis E virus (HEV) infection in individuals who are immunocompromised and are at risk for or suspected to have acute or chronic hepatitis E

 

Monitoring HEV RNA levels and determining eradication of chronic HEV infection in individuals who are immunocompromised

Testing Algorithm

For more information see the following algorithms:

-Hepatitis E: Diagnostic Testing Algorithm

-Hepatitis D: Diagnostic Testing Algorithm

Method Name

Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

Reporting Name

HEV RNA Detect / Quant, S

Specimen Type

Serum SST

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Frozen (preferred) 35 days ALIQUOT TUBE
  Refrigerated  5 days ALIQUOT TUBE

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

Undetected

Method Description

This assay utilizes real-time polymerase chain reaction (PCR) technology for the qualitative and quantitative detection of hepatitis E virus (HEV) RNA in human serum. Testing involves 3 major processes with the ELITe InGenius integrated system: automated extraction and purification of viral RNA; reverse transcription of viral target RNA sequence to generate complementary DNA (cDNA); and PCR amplification of a 69-base pair sequence in the 5' end of the HEV open reading frame (ORF) 2 that encodes the viral capsid protein, followed by real-time detection of fluorescent dye-labeled oligonucleotide probes that allow the specific and simultaneous detection and quantification of the target sequence as well as an MS2 internal control. The assay is calibrated to the First World Health Organization International Standard for HEV RNA, PEI code 6329/10.(Germer JJ, Ankoudinova I, Belousov YS, et al. Hepatitis E virus detection and quantification by an RT-PCR assay calibrated to the World Health Organization Standard for HEV RNA. J Clin Microbiol. 2017;55[5]:1478-1487. doi:10.1128/JCM.02334-16)

Day(s) Performed

Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87799

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HEVQU HEV RNA Detect / Quant, S 69961-1

 

Result ID Test Result Name Result LOINC Value
62929 HEV RNA Detect / Quant, S 69961-1

Forms

If not ordering electronically, complete, print, and send 1 of the following with the specimen:

-Gastroenterology and Hepatology Test Request (T728)

-Microbiology Test Request (T244)