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Order Code HICSF Histoplasma Antibody Complement Fixation and Immunodiffusion, Spinal Fluid


Ordering Guidance


This test should be ordered if patient is seropositive for Histoplasma antibodies in serum.



Specimen Required


Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Submit specimen from collection vial number1.


Useful For

Aiding in the diagnosis of Histoplasma meningitis using spinal fluid specimens

Testing Algorithm

For information see Meningitis/Encephalitis Panel Algorithm

Method Name

Complement Fixation (CF)/Immunodiffusion (ID)

Reporting Name

Histoplasma Ab CompFix/ImmDiff, CSF

Specimen Type

CSF

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time
CSF Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

Anti-Yeast Antibody by Complement Fixation: Negative

 

Antibody by Immunodiffusion: Negative

Method Description

Both immunodiffusion and compliment fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen is a culture filtrate, histoplasmin. H and M precipitin bands are identified. For the CF test, the antigens are histoplasmin and a yeast form of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD. Fungi. In: Washington II, JA eds. Laboratory Procedures in Clinical Microbiology. 2nd ed. Springer-Verlag; 1985; Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86698 x2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HICSF Histoplasma Ab CompFix/ImmDiff, CSF 91684-1

 

Result ID Test Result Name Result LOINC Value
621217 Histoplasma Yeast CompFix, CSF 27209-6
621218 Histoplasma Immunodiffusion, CSF 91682-5

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.