Home
HelpOrder Code HISER Histoplasma Antibody Complement Fixation and Immunodiffusion, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
Aiding in the diagnosis of active histoplasmosis
Testing Algorithm
For more information see Meningitis/Encephalitis Panel Algorithm.
Special Instructions
Method Name
Complement Fixation (CF)/Immunodiffusion (ID)
Reporting Name
Histoplasma Ab CompFix/ImmDiff, SSpecimen Type
SerumSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
Anti-Yeast Antibody by Complement Fixation:
Negative (positive results reported as titer)
Antibody by Immunodiffusion:
Negative (positive results reported as band present)
Method Description
Both immunodiffusion and complement fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen used is a culture filtrate. Histoplasmin H and M precipitins can be identified by the assay. For the CF test, antigens are histoplasmin and a yeast form antigen of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD. Fungi. In: Washington II JA, ed. Laboratory Procedures in Clinical Microbiology. 2nd ed. Springer-Verlag, 1985; Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86698 x2