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HelpOrder Code IBDP2 Inflammatory Bowel Disease Serology Panel, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Distinguishing between ulcerative colitis and Crohn disease in patients for whom the specific diagnosis is unclear based on endoscopic, pathologic, and imaging evaluations
This test is not useful for determining the extent of disease in patients with inflammatory bowel disease or determining the response to disease-specific therapy including surgical resection of diseased intestine.
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANCA2 | Cytoplasmic Neutrophilic Ab IBD, S | No | Yes |
| SCERA | Saccharomyces cerevisiae Ab, IgA, S | Yes | Yes |
| SCERG | Saccharomyces cerevisiae Ab, IgG, S | Yes | Yes |
Method Name
SCERA, SCERG: Enzyme-Linked Immunosorbent Assay (ELISA)
ANCA2: Indirect Immunofluorescent Assay (IFA)
Reporting Name
Inflammatory Bowel Disease Panel, SSpecimen Type
SerumSpecimen Minimum Volume
0.8 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimen | Reject |
Reference Values
Saccharomyces cerevisiae ANTIBODY, IgA
Negative: <20.0 RU/mL
Positive: ≥20.0 RU/mL
Saccharomyces cerevisiae ANTIBODY, IgG
Negative: <20.0 RU/mL
Positive: ≥20.0 RU/mL
CYTOPLASMIC NEUTROPHIL ANTIBODIES, INFLAMMATORY BOWEL DISEASE PANEL, SERUM
Negative (not detectable)
Method Description
IgA or IgG antibodies to Saccharomyces cerevisiae antigens (ASCA) are measured by commercial, microtiter enzyme immunoassays. These assays use polystyrene microtiter plates coated with purified mannan from the cell wall of S cerevisiae to capture antibodies from patient sera, and horseradish peroxidase-conjugated anti-IgA or anti-IgG antibodies to detect ASCA. Results of the tests for ASCA are reported in relative units per milliliter (RU/mL).(Package inserts: Anti-Saccharomyces cerevisiae ELISA (IgA), 5/2011; Anti-Saccharomyces cerevisiae ELISA (IgG), EUROIMMUN Medizinische Labordiagnostika AG; 5/2011)
Antineutrophil cytoplasmic antibodies (ANCA) are detected qualitatively using an in-house developed method with a substrate of ethanol-fixed, human neutrophils and fluorescein conjugated, antihuman IgG antibody as a detection protein. Results of the test for ANCA are reported as positive, negative, or indeterminate.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86671 x 2
86036
Forms
If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen.
Testing Algorithm
For more information see Inflammatory Bowel Disease Diagnostic Testing Algorithm.
For more information see Ulcerative Colitis and Crohn Disease Therapeutic Drug Monitoring Algorithm.