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HelpOrder Code IFXED Immunofixation Heavy Chain Type Delta and Epsilon, Serum
Specimen Required
Patient Preparation: Fasting preferred but not required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Identification and isotyping of IgD or IgE monoclonal gammopathy
Documentation of complete response to therapy with established monoclonal gammopathy consisting of an IgD or IgE heavy chain
Method Name
Immunofixation
Reporting Name
Immunofixation Delta and Epsilon, SSpecimen Type
SerumSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 14 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Immunofixation Delta and Epsilon: No monoclonal IgD or IgE protein detected.
Immunofixation Delta and Epsilon, Flag: Negative
Method Description
Immunofixation is performed with reagents specific for delta and epsilon immunoglobulin heavy chains and kappa and lambda light chains. Immunofixation electrophoresis is performed in four stages: 1) separation of proteins by electrophoresis on an agarose gel; 2) immunofixation (immunoprecipitation) and fixation of the electrophoresed proteins;3) removal of unprecipitated soluble proteins by blotting and washing; and 4) staining of the precipitated proteins for visualization.(Package insert: HYDRAGEL 1, 2, 4 and 9 IF kit. Sebia Inc; 07/2020)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86334
Day(s) Performed
Monday through Friday