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HelpOrder Code KLHCC Kelch-Like Protein 11 Antibody, Cell Binding Assay, Spinal Fluid
Specimen Required
Patient Preparation: For optimal antibody detection, specimen collection is recommended before initiation of immunosuppressant medication, or corticosteroid or intravenous immunoglobulin (IVIg) treatment.
Container/Tube: Sterile vial
Preferred: Collection vial number 1
Acceptable: Any collection vial
Specimen Volume: 1 mL
Useful For
Evaluating patients with paraneoplastic or autoimmune encephalitis (brainstem encephalitis or limbic encephalitis or cerebellar ataxia) using spinal fluid specimens
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| K11TC | KLHL11 Ab IFA Titer, CSF | No | No |
Testing Algorithm
If the cell binding antibody result is reactive, then the immunofluorescence titer assay will be performed at an additional charge.
Method Name
Cell-Binding Assay (CBA)
Reporting Name
KLHL11-IgG CBA, CSFSpecimen Type
CSFSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| CSF | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Reference Values
Negative
Method Description
Patient specimen is applied to a composite slide containing transfected and nontransfected EU90 cells. After incubation and washing, fluorescein-conjugated goat-antihuman IgG is applied to detect the presence of patient IgG binding. (Package insert: EUROIMMUN; FA_110a-50_A_UK_B01, 12/2023)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
0432U