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Order Code KRASD Cell-Free DNA KRAS 12, 13, 61,146, Blood


Ordering Guidance


This test is not a prenatal screening test



Shipping Instructions


1. Samples should be transported at ambient temperature or refrigerated (4° C).

2. Samples are viable for 7 days in the Streck Black/Tan Top Tube Kit (T715).



Specimen Required


Supplies: Streck Black/Tan Top Tube Kit (T715)

Container/Tube: Streck Cell-Free DNA blood collection kit

Specimen Volume: Two 10-mL Streck Cell-Free DNA blood collection tubes

Additional Information: Only blood collected in Streck Cell-Free DNA BCT tubes will be accepted for analysis. Whole blood will be processed to produce platelet-poor plasma before cfDNA isolation.


Useful For

An alternative to invasive tissue biopsies for the determination of KRAS 12, 13, 61,146 (G12A, G12C, G12D, G12R, G12S, G12V, G13D, Q61K, Q61L, Q61R, Q61H, and A146T) mutation status

 

Detecting molecular markers associated with response or resistance to specific therapy

 

This test is not intended as a screening test to identify cancer.

Genetics Test Information

This test evaluates cell-free DNA (cfDNA) in the peripheral blood for the presence of KRAS mutations at codons 12, 13, 61, and 146 (G12A, G12C, G12D, G12R, G12S, G12V, G13D, Q61K, Q61L, Q61R, Q61H, and A146T) in patients with cancer and can be used to assess eligibility for targeted therapies.

Method Name

Digital Droplet Polymerase Chain Reaction (PCR)

Reporting Name

cfDNA KRAS 12, 13, 61, 146 Blood

Specimen Type

Whole blood

Specimen Minimum Volume

One 10 mL Streck tube

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred) 7 days Streck Black/Tan top
  Refrigerated  7 days Streck Black/Tan top

Reject Due To

Specimen collected in tube other than Streck Cell-Free DNA collection tube Reject

Method Description

Blood samples are collected in Streck Cell-Free DNA BCT tubes. Cell-free DNA (cfDNA) is isolated from double-spun plasma and assessed for the presence of KRAS codon 12, 13, 61, and 146 mutations using droplet digital polymerase chain reaction.(Unpublished Mayo method)

Day(s) Performed

Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81275

81276

 

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.

Reference Values

An interpretive report will be provided