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Order Code LAB1205 Aldolase, Serum

Additional Codes

Mayo Test Code: ALS

Reporting Name

Aldolase, S

Useful For

Detection of muscle disease

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Specimen Required


Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Within 1 hour of collection, centrifuge and aliquot serum into a plastic vial.

2. Send refrigerated.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 7 days
  Frozen  60 days

Reference Values

<18 years: <14.5 U/L

≥18 years: <7.7 U/L

Day(s) Performed

Monday through Sunday

CPT Code Information

82085

Method Description

The aldolase activity is determined from the rate at which reduced nicotinamide adenine dinucleotide (NADH) is oxidized to NAD(+) and is measured photometrically by a decrease in absorbance. For each mole of substrate hydrolyzed, 2 moles of coenzyme (NADH) are oxidized. This 1:2 ratio is applied in the calculation of aldolase activity. Lactate dehydrogenase is present in the reagent for the purpose of eliminating interference from endogenous pyruvate in the specimen.(Package insert: Aldolase Reagent. Roche Diagnostics; V 5.0, 01/2021)

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Photometric

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.