Order Code LAB1311 5-Hydroxyindoleacetic Acid, 24 Hour, Urine
Reporting Name
5-Hydroxyindoleacetic Acid, UUseful For
Biochemical diagnosis and monitoring of intestinal carcinoid syndrome using 24-hour urine specimens
Performing Laboratory
Mayo Clinic Laboratories in RochesterSpecimen Type
UrineOrdering Guidance
This test is the preferred test for measurement of 5-hydroxyindoleacetic acid concentrations. If a random urine collection is preferred, order HIAAR / 5-Hydroxyindoleacetic Acid, Random, Urine.
Necessary Information
1. Collection duration and urine volume in milliliters are required.
2. Patient's age and sex are required.
Specimen Required
Patient Preparation:
1. Some medications could interfere with test results. The ordering provider should decide if any medications should be stopped and when they should be restarted. If clinically feasible, discontinue the following medications at least 48 hours prior to, as well as during, specimen collection:
-Acetaminophen (Tylenol or generic versions)
-Tryptophan containing supplements
2. For 48 hours prior to, as well as during, the urine collection, the patient should:
Limit the following to one serving per day:
-Fruits
-Vegetables
-Nuts
-Caffeinated beverages or foods
Supplies: Urine Tubes, 10 mL (T068)
Container/Tube: Plastic, 10-mL urine tube
Specimen Volume: 5 mL
Collection Instructions:
1. Add 25 mL of 50% acetic acid as preservative at start of collection. Use 15 mL of 50% acetic acid for children <5 years old.
2. Collect a 24-hour urine specimen.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Specimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Refrigerated (preferred) | 56 days | |
Frozen | 365 days |
Special Instructions
Reference Values
5-HIAA, mg/24 hour 99th percentile cutoff |
||
Age |
Female |
Male |
≤23 months |
≤2.7 |
≤2.3 |
24-35 months |
≤3.0 |
≤2.6 |
3 years |
≤3.2 |
≤2.9 |
4 years |
≤3.4 |
≤3.2 |
5 years |
≤3.6 |
≤3.6 |
6 years |
≤3.8 |
≤3.9 |
7 years |
≤4.0 |
≤4.2 |
8 years |
≤4.2 |
≤4.5 |
9 years |
≤4.5 |
≤4.8 |
10 years |
≤4.7 |
≤5.1 |
11 years |
≤4.9 |
≤5.3 |
12 years |
≤5.2 |
≤5.6 |
13 years |
≤5.4 |
≤5.8 |
14 years |
≤5.6 |
≤6.1 |
15 years |
≤5.7 |
≤6.3 |
16 years |
≤5.9 |
≤6.4 |
17 years |
≤6.0 |
≤6.6 |
18 years |
≤6.0 |
≤6.7 |
19 years |
≤6.1 |
≤6.8 |
20 years |
≤6.1 |
≤6.9 |
21 years |
≤6.2 |
≤6.9 |
22 years |
≤6.2 |
≤7.0 |
23 years |
≤6.2 |
≤7.0 |
24 years |
≤6.3 |
≤7.1 |
25 years |
≤6.3 |
≤7.2 |
26 years |
≤6.3 |
≤7.2 |
27 years |
≤6.4 |
≤7.3 |
28 years |
≤6.4 |
≤7.4 |
29 years |
≤6.5 |
≤7.5 |
30 years |
≤6.6 |
≤7.5 |
31 years |
≤6.6 |
≤7.6 |
32 years |
≤6.7 |
≤7.7 |
33 years |
≤6.8 |
≤7.7 |
34 years |
≤6.8 |
≤7.8 |
35 years |
≤6.9 |
≤7.9 |
36 years |
≤6.9 |
≤7.9 |
37 years |
≤7.0 |
≤8.0 |
38 years |
≤7.0 |
≤8.1 |
39 years |
≤7.0 |
≤8.2 |
40 years |
≤7.1 |
≤8.2 |
41 years |
≤7.1 |
≤8.3 |
42 years |
≤7.2 |
≤8.4 |
43 years |
≤7.3 |
≤8.5 |
44 years |
≤7.4 |
≤8.6 |
45 years |
≤7.4 |
≤8.7 |
46 years |
≤7.5 |
≤8.8 |
47 years |
≤7.6 |
≤8.9 |
48 years |
≤7.6 |
≤9.0 |
49 years |
≤7.7 |
≤9.1 |
50 years |
≤7.7 |
≤9.2 |
51 years |
≤7.8 |
≤9.3 |
52 years |
≤7.8 |
≤9.4 |
53 years |
≤7.9 |
≤9.5 |
54 years |
≤8.0 |
≤9.6 |
55 years |
≤8.1 |
≤9.7 |
56 years |
≤8.1 |
≤9.7 |
57 years |
≤8.2 |
≤9.8 |
58 years |
≤8.3 |
≤9.8 |
59 years |
≤8.3 |
≤9.8 |
60 years |
≤8.3 |
≤9.9 |
61 years |
≤8.3 |
≤9.9 |
62 years |
≤8.4 |
≤9.9 |
63 years |
≤8.4 |
≤10.0 |
64 years |
≤8.4 |
≤10.0 |
65 years |
≤8.4 |
≤10.0 |
66 years |
≤8.5 |
≤10.1 |
67 years |
≤8.5 |
≤10.1 |
68 years |
≤8.5 |
≤10.1 |
69 years |
≤8.5 |
≤10.2 |
70 years |
≤8.5 |
≤10.2 |
71 years |
≤8.6 |
≤10.2 |
72 years |
≤8.6 |
≤10.2 |
73 years |
≤8.5 |
≤10.1 |
74 years |
≤8.5 |
≤10.1 |
75 years |
≤8.6 |
≤10.1 |
76 years |
≤8.6 |
≤10.0 |
77 years |
≤8.6 |
≤10.0 |
78 years |
≤8.6 |
≤10.0 |
79 years |
≤8.6 |
≤10.0 |
80 years |
≤8.7 |
≤9.9 |
81 years |
≤8.7 |
≤9.9 |
82 years |
≤8.7 |
≤9.9 |
83 years |
≤8.7 |
≤9.9 |
84 years |
≤8.7 |
≤9.9 |
85 years |
≤8.6 |
≤9.8 |
86 years |
≤8.5 |
≤9.8 |
87 years |
≤8.4 |
≤9.7 |
88 years |
≤8.3 |
≤9.7 |
89 years |
≤8.1 |
≤9.5 |
90 years |
≤7.9 |
≤9.4 |
91 years |
≤7.6 |
≤9.2 |
92 years |
≤7.4 |
≤9.0 |
93 years |
≤7.1 |
≤8.8 |
94 years |
≤7.0 |
≤8.7 |
= 95 years |
≤6.9 |
≤8.6 |
For SI unit Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Day(s) Performed
Monday through Friday
CPT Code Information
83497
Method Description
5-Hydroxyindoleacetic acid (5-HIAA) is measured by solid phase extraction of an aliquot from a 24-hour urine collection and liquid chromatography tandem mass spectrometry analysis. 5-HIAA is quantitated using a custom synthesized stable isotope labeled internal standard (d6-5-HIAA) from calibration over a concentration range 0.5 to 150 mg/L.(Kroll CA, Magera MJ, Helgeson JK, Mattern D, Rinaldo P. A liquid chromatography-tandem mass spectrometry method for the determination of 5-hydroxyindole-3-acetic acid in urine. Clin Chem. 2002;48:2049-2051; Calanchini M, Tadman M, Krogh J, Fabbri A, Grossman A, Shine B. Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection. Endocr Connect. 2019;8[8]:1082-1088)
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.