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Order Code LAB1324 Immunoglobulin E (IgE), Serum

Additional Codes

Mayo Test ID
IGE

Reporting Name

Immunoglobulin E (IgE), S

Useful For

Evaluating patients with suspected diseases associated with elevations in total immunoglobulin E (IgE), including allergic disease, primary immunodeficiencies, infections, malignancies, or other inflammatory diseases 

 

Diagnostic evaluation of patients with suspected allergic bronchopulmonary aspergillosis

 

 Identifying candidates for omalizumab (anti-IgE) therapy

Specimen Type

Serum


Ordering Guidance


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

For total IgE: 0.3 mL
For total IgE and more than 1 allergen: 0.05 mL x number of allergen-specific tests + 0.25 mL dead space

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  90 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

Results reported in kU/L

Age

Reference interval

0-5 months

≤13

6-11 months

≤34

1 and 2 years

≤97

3 years

≤199

4-6 years

≤307

7 and 8 years

≤403

9-12 years

≤696

13-15 years

≤629

16 and 17 years

≤537

18 years and older

≤214

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Friday

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

CPT Code Information

82785

Method Description

Anti-immunoglobulin E (IgE), covalently coupled to ImmunoCAP, reacts with the IgE in a serum specimen. After washing, enzyme-labeled anti-IgE antibodies are added to form a complex. After incubation, unbound enzyme-labeled anti-IgE is washed away and the bound complex is incubated with a developing agent. After stopping the reaction, fluorescence of the eluate in the well is measured. The fluorescence is directly proportional to the concentration of IgE in the test specimen.(Package insert: Phadia CAP System IgE FEIA. Phadia; revised 04/2019)