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HelpOrder Code LAB1398 T3 (Triiodothyronine), Free, Serum
Additional Codes
Mayo Test Code: T3FR
EPIC Test Code: LAB1398 (back-up for in-house Free T3)
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
A second- or third-level test of thyroid function
Confirmation of hyperthyroidism, supplementing the T4 (tetraiodothyronine), sensitive thyrotropin, and total T3 assays
Evaluating clinically euthyroid patients who have an altered distribution of binding proteins
Method Name
Electrochemiluminescence Immunoassay (ECLIA)
Reporting Name
T3 (Triiodothyronine), Free, SSpecimen Type
SerumSpecimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Pediatric
0-1 month: 2.7-8.5 pg/mL
1-<12 months: 3.4-5.6 pg/mL
1-<14 years: 3.0-5.1 pg/mL
14-<19 years: 3.3-5.3 pg/mL
Adult (≥19 years): 2.0-4.4 pg/mL
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html.
Method Description
The Roche Elecsys FT3 III (free T3 [triiodothyronine]) is a competitive assay using electrochemiluminescence detection. The patient sample is incubated with a sheep polyclonal anti-T3 antibody labeled with ruthenium. Streptavidin-coated microparticles and biotinylated T3 are added for a second incubation during which the still free binding sites of the labeled antibody become occupied. The resulting immunocomplex becomes bound to the solid phase by interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are removed and application of a voltage to the electrode induces the electrochemiluminescent emission. This signal is measured against a calibration curve to determine patient results.(Package insert: Elecsys FT3 III. Roche Diagnostics; 10/2022)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84481