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Order Code LAB1399 T3 (Triiodothyronine), Total, Serum

Reporting Name

T3 (Triiodothyronine), Total, S

Useful For

Second-order testing for hyperthyroidism in patients with low thyroid-stimulating hormone values and normal thyroxine levels

 

Diagnosing triiodothyronine (T3) toxicosis

 

This test is not useful for general screening of the population without clinical suspicion of hyperthyroidism.

Testing Algorithm

For information see Thyroid Function Ordering Algorithm.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  30 days

Reference Values

Pediatric

0-5 days: 73-288 ng/dL

6 days-2 months: 80-275 ng/dL

3-11 months: 86-265 ng/dL

1-5 years: 92-248 ng/dL

6-10 years: 93-231 ng/dL

11-19 years: 91-218 ng/dL

 

Adult (≥20 years): 80-200 ng/dL

 

For SI unit Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) Performed

Monday through Saturday

CPT Code Information

84480

Method Description

The Roche triiodothyronine assay (T3) is a competitive assay using electrochemiluminescence detection. Bound T3 is released from binding proteins by 8-anilino-1-naphthalene sulfonic acid. The patient specimen is incubated with a sheep polyclonal anti-T3 antibody labeled with ruthenium. Streptavidin-coated microparticles and biotinylated T3 are added for a second incubation, during which the still free binding sites of the labeled antibody become occupied. The resulting immunocomplex becomes bound to the solid phase by interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are removed, and application of a voltage to the electrode induces the electrochemiluminescent emission. This signal is measured against a calibration curve to determine patient results.(Package insert: Elecsys T3. Roche Diagnostics; 08/2019)

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Electrochemiluminescence Immunoassay