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HelpOrder Code LAB1413 Diazepam and Nordiazepam, Serum
Additional Codes
Mayo Test Code: DIA
Test Down Notes
Effective December 14, 2023: This test is temporarily unavailable due to analytic issues. The downtime is expected to be >30 days. Order ZW86 (1501SP) as an alternate. See test notification here.
Reporting Name
Diazepam and Nordiazepam, SUseful For
Assessing compliance
Monitoring for appropriate therapeutic level
Assessing diazepam toxicity
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedSpecimen Required
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 14 days | |
| Ambient | 14 days | ||
| Frozen | 14 days |
Reference Values
Therapeutic concentrations
Diazepam and Nordiazepam: 200-2,500 ng/mL
Day(s) Performed
Monday, Wednesday
CPT Code Information
80299
Method Description
The internal standard mixture containing chlordiazepoxide-d5, diazepam-d4, and nordiazepam-d5 is added to serum samples. The serum samples are treated with phosphate buffer and extracted via liquid/liquid extraction. The organic layer from the extraction is dried under nitrogen, reconstituted, and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Neurology Specialty Testing Client Test Request (T732)
-Therapeutics Test Request (T831)