Sign in →

Order Code LAB1822 Trichinella Antibody, IgG, Serum

Test Down Notes

Effective October 17, 2025: This test is obsolete due to unavailability of testing reagent. Order ZW131, 34321 as an alternative. See test notification here.


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Useful For

As an adjunct in the diagnosis of trichinosis

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Trichinella Ab, IgG, S

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 30 days
  Refrigerated  5 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat-inactivated Reject

Reference Values

Negative

Reference values apply to all ages.

Method Description

Microtiter strip wells are precoated with Trichinella spiralis ES-antigens (excretory/secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase-labelled protein A conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow end point color. Absorbance alternative 450 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Trichinella spiralis ELISA IgG. Gold Standard Diagnostics; 11/02/2021)

Day(s) Performed

Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86784

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TRCNG Trichinella Ab, IgG, S 6563-1

 

Result ID Test Result Name Result LOINC Value
TRCNG Trichinella Ab, IgG, S 19253-4

Forms

If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.