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HelpOrder Code LAB1822 Trichinella Antibody, IgG, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
As an adjunct in the diagnosis of trichinosis
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Trichinella Ab, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 30 days |
| Refrigerated | 5 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated | Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
Microtiter strip wells are precoated with Trichinella spiralis ES-antigens (excretory/secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase-labelled protein A conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow end point color. Absorbance alternative 450 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Trichinella spiralis ELISA IgG. Gold Standard Diagnostics; 11/02/2021)
Day(s) Performed
Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86784
Forms
If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.