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HelpOrder Code LAB1905 C5 Complement, Antigen, Serum
Reporting Name
C5 Complement, Antigen, SUseful For
Diagnosis of C5 deficiency
Investigation of a patient with an absent total complement (CH50) level
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumOrdering Guidance
The total complement assay (COM / Complement, Total, Serum) should be used as a screen for suspected complement deficiencies before ordering individual complement component assays. A deficiency of an individual component of the complement cascade will result in an undetectable total complement level.
Specimen Required
Patient Preparation: Fasting preferred but not required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and separate serum from clot.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 60 days | |
| Refrigerated | 28 days | ||
| Ambient | 7 days |
Reference Values
10.6-26.3 mg/dL
Day(s) Performed
Monday through Friday
CPT Code Information
86160
Method Description
C5 complement antigen is measured by immunonephelometry. Antiserum to C5 is mixed with patient serum, the light scatter resulting from the antibody interaction with C5 is measured, and the signal is compared to standard concentrations of C5.(Unpublished Mayo information; Instruction manual: Siemens Nephelometer II l Version 3, Siemens, Inc., Newark, DE, 2008)
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
Nephelometry