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Order Code LAB1951 Cancer Antigen 125 (CA 125), Serum

Reporting Name

Cancer Ag 125 (CA 125), S

Useful For

Evaluating individuals' response to ovarian cancer therapy

 

Predicting recurrent ovarian cancer

 

This test is not useful for cancer detection screening in the normal population.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 5 days
  Frozen  168 days

Reference Values

Males: Not applicable

Females: <46 U/mL

Day(s) Performed

Monday through Friday

CPT Code Information

86304

Method Description

The Roche Elecsys CA 125 II (cancer antigen 125) assay is a sandwich electrochemiluminescence immunoassay that employs a biotinylated monoclonal CA 125-specific antibody and a monoclonal CA 125-specific antibody labeled with a ruthenium complex. CA 125 in the specimen reacts with both the biotinylated monoclonal CA 125-specific antibody (mouse) and the monoclonal CA 125-specific antibody (mouse) labeled with ruthenium, forming a sandwich complex. Streptavidin-coated microparticles are added and the mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of voltage to the electrode induces the chemiluminescent emission, which is then measured against a calibration curve to determine the amount of CA 125 in the patient specimen.(Package insert: Elecsys CA 125 II. Roche Diagnostics; V1.0, 09/2020)

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Method Name

Electrochemiluminescent Immunoassay (ECLIA)

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.