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HelpOrder Code LAB2758 Culture Referred for Identification, Fungus
Infectious
Additional Codes
| Mayo Test ID |
|---|
| FUNID |
Reporting Name
Culture Referred for ID, FungusUseful For
Identification of pure isolates of filamentous fungi and yeast
Specimen Type
VariesShipping Instructions
1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
Specimen Required
Specimen Type: Organism in pure culture
Supplies: Infectious Container, Large (T146)
Container/Tube:
Preferred: Sabouraud dextrose agar slant
Acceptable: Inhibitory mold agar slant
Specimen Volume: Isolated mold or yeast
Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Agar plate | Reject |
Reference Values
Not applicable
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Saturday
Method Name
Macroscopic/Microscopic/D2 rDNA Gene Sequencing/Real-Time Polymerase Chain Reaction (rtPCR)/Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)
Dimorphic Pathogen Identification Confirmation: D2 rDNA Gene Sequencing/rtPCR/MALDI-TOF MS
CPT Code Information
87107-Culture, fungi, definitive identification
87106-Culture, fungi, definitive identification, each organism; yeast (if appropriate)
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Culture, fungi, definitive identification, each organism; mold (if appropriate)
87107-Fungal identification Panel A (if appropriate)
87107-Fungal identification Panel B (if appropriate)
87150-Identification rapid PCR coccidioides (if appropriate)
87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)
87153-D2 fungal sequencing Identification (if appropriate)
87150-Id, Candida auris Rapid PCR (if appropriate)
Special Instructions
Method Description
Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, laboratory-developed real-time polymerase chain reaction (PCR) assays and/or D2 rDNA gene sequencing, as applicable.(Babady NE, Buckwalter SP, Hall L, Le Febre KM, Binnicker MJ, Wengenack NL: Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR. J Clin Microbiol. 2011 Sep;49[9]:3204-3208; Binnicker MJ, Buckwalter SP, Eisberner JJ, et al: Detection of Coccidioides species in clinical specimens by real-time PCR. J Clin Microbiol. 2007 Jan;45[1]:173-178; Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL: Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011 Apr;49[4]:1614-1616; Hall L, Wohlfiel SL, Roberts GD: Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004 Feb;42[2]:622-626; Theel ES, Schmidt BH, Hall L, et al: Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012 Sep;50[9]:3093-3095; Theel ES, Hall L, Mandrekar J, Wengenack NL: Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011 Dec;49[12]:4067-4071)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| LCCI | Ident Rapid PCR Coccidioides | No, (Bill Only) | No |
| LCHB | Id, Histoplasma/Blastomyces PCR | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
| LCCA | Id, Candida auris Rapid PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed and charged. All fungal organisms submitted will be identified and billed as appropriate.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.