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HelpOrder Code LAB3282 Metanephrines, Fractionated, Free, Plasma
Additional Codes
| Mayo Test ID |
|---|
| PMET |
Reporting Name
Metanephrines, Fract., Free, PUseful For
Screening test for presumptive diagnosis of catecholamine-secreting pheochromocytomas or paragangliomas
Specimen Type
Plasma EDTASpecimen Required
Patient Preparation: Use of an Epi-pen within the last 7 days may produce inaccurate results.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Within 2 hours of collection centrifuge and aliquot plasma into a plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Frozen (preferred) | 28 days |
| Refrigerated | 14 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
METANEPHRINE, FREE
<0.50 nmol/L
NORMETANEPHRINE, FREE
<0.90 nmol/L
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Saturday
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
CPT Code Information
83835
Method Description
Free metanephrine (MN) and normetanephrine (NMN) are extracted from plasma using solid phase extraction. The concentrated eluate is analyzed using liquid chromatography tandem mass spectrometry and quantified using stable isotope labeled internal standards, d3-MN and d3-NMN . Analytes and internal standards are ionized using electro spray ionization and are detected in the multiple reaction-monitoring mode. The specific transitions for MN, NMN, d3-MN, and d3-NMN are m/z 180.2 to m/z 148.2, 166.2 to m/z 134.2, 183.2 to m/z 151.2, and m/z 169.2 to m/z 137.2, respectively.(Unpublished Mayo method)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Oncology Test Request (T729)
-Renal Diagnostics Test Request (T830)