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HelpOrder Code LAB4144 Vitamin B3 and Metabolites, Plasma
Specimen Required
Patient Preparation: Fasting 4 to 8 hours preferred
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Lavender top (K2 EDTA)
Acceptable: K3 EDTA
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial within 2 hours of collection.
Useful For
Assisting in the diagnosis of suspected vitamin B3 deficiency or toxicity
May be useful in determining response to therapy
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin B3 and Metabolites, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Nicotinic Acid (Niacin) Cutoff: <5.0 ng/mL
Nicotinamide: 5.0-48.0 ng/mL
Nicotinuric Acid Cutoff: <5.0 ng/mL
Method Description
Vitamin B3 components (nicotinic acid/niacin, nicotinamide, nicotinuric acid) are extracted from plasma specimens with internal standard and then analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Monday, Tuesday, Thursday, Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84591