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HelpOrder Code LAB4568 Cytomegalovirus (CMV) Molecular Detection, PCR, Varies
Additional Codes
Mayo Test Code: CMVPV
Ordering Guidance
For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.
Necessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular
Supplies: Sarstedt Aliquot Tube, 5mL (T914)
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory fluid
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Supplies: Sarstedt Aliquot Tube, 5mL
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 1.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Genital swab
Sources: Cervix, vagina, urethra, anal/rectal, or other genital sources
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT) and ESwabs
Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)
Specimen Type: Swab
Sources: Dermal, eye, nasal, saliva, or throat
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT) and ESwabs
Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, etc.
Supplies: M4-RT (T605)
Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) or a sterile container with 1 to 2 mL sterile saline
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 0.5 mL
Collection Instructions: Send bone marrow in original tube. Do not aliquot.
Useful For
Rapid qualitative detection of cytomegalovirus (CMV) DNA
This test is not intended for the monitoring of CMV disease progression.
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reporting Name
Cytomegalovirus, PCRSpecimen Type
VariesSpecimen Minimum Volume
Ocular Fluid, Spinal Fluid, or Urine: 0.3 mL
Body Fluid (pleural, peritoneal, ascites, pericardial): See Specimen Required
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Calcium alginate-tipped swab Wood swab Transport swab containing gel Feces Paraffin blocks Breast milk Heat inactivated specimens |
Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
Viral nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science) from clinical specimens. Primers directed to the target Us9 gene produce a 285-base pair amplicon. The LightCycler instrument amplifies and monitors by fluorescence the development of target nucleic acid sequences after the annealing step during polymerase chain reaction (PCR) cycling. This is an automated PCR system that can rapidly detect amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the fluorescence resonance energy transfer (FRET) principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5'-end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product. Melting curve analysis is performed following PCR amplification. Starting at 45° C, the temperature in the thermal chamber is slowly raised to 80° C and the fluorescence is measured at frequent intervals. Analysis of the PCR amplification and probe melting curves is accomplished through the use of LightCycler software.(Binnicker MJ, Espy M. Comparison of six real-time PCR assays for the qualitative detection of cytomegalovirus in clinical specimens. J Clin Microbiol. 2013:51[11]:3749-3752)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87496
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.