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HelpOrder Code LAB5036 Hepatitis B Virus Core IgM Antibody, Serum
Additional Codes
| Mayo Test ID |
|---|
| HBIM |
Reporting Name
HBc IgM Ab, SUseful For
Diagnosis of acute hepatitis B virus (HBV) infection
Identifying acute HBV infection in the serologic window period when HBV surface antigen and HBV surface antibody results are negative
Differentiation between acute, chronic, or past HBV infections in the presence of positive hepatitis B virus core total antibodies
Specimen Type
Serum SSTNecessary Information
Date of collection is required.
Specimen Required
Patient Preparation: For 24 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Serum gel (red-top tubes are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions:
1. Centrifuge blood collection tube per manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Aliquot serum into a plastic tube.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum SST | Frozen (preferred) | 90 days |
| Refrigerated | 6 days | |
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Saturday
Method Name
Electrochemiluminescence Immunoassay (ECLIA)
CPT Code Information
86705
Special Instructions
Method Description
The Elecsys Anti-HBc (hepatitis B virus core antibody) IgM assay is based on the sandwich immunoassay principle and performed with an electrochemiluminescence immunoassay on the automated cobas e 801 immunochemistry analyzer. Anti-HBc IgM present in patient's sample is pretreated with anti-Fdy reagent to block specific IgG. After addition of biotinylated monoclonal human IgM-specific antibodies, the complexes formed from reaction of ruthenium-labeled HBc antigen, streptavidin-coated microparticles (solid phase), anti-HBc IgM present in the sample, and the biotinylated anti-human IgM bind to the solid phase via interaction of biotin and streptavidin. The reaction mixture is then aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode, and unbound substances are washed away. Voltage is applied to the electrode that induces chemiluminescent emissions, which are measured by a photomultiplier. Test result is determined by comparing the electrochemiluminescence signal generated from the sample to the cutoff index value set from reagent lot-specific assay calibration.(Package insert: Elecsys Anti-HBc IgM. Roche Diagnostics; v2.0, 12/2024)
Forms
If not ordering electronically, complete, print, and send 1 of the following:
Testing Algorithm
For more information see Hepatitis B: Testing Algorithm for Screening, Diagnosis, and Management.