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HelpOrder Code LAB5063 Measles (Rubeola) Antibodies, IgM, Serum
Additional Codes
EPIC Tets Code: LAB5063
| Mayo Test ID |
|---|
| MEAM |
Reporting Name
Measles (Rubeola) Ab, IgM, SUseful For
Determining acute-phase infection with rubeola (measles) virus using IgM antibody testing
Aiding in the identification of nonimmune individuals through IgM antibody testing
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Gross reject |
| Gross lipemia | Gross reject |
| Heat-inactivated specimen | Reject |
Reference Values
Negative
Reference values apply to all ages.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Saturday
Method Name
Immunofluorescence Assay (IFA)
CPT Code Information
86765
Method Description
The presence of IgM-class antibody to measles is determined by an indirect immunofluorescence assay. After removal of IgG by specific immunoglobulin antibody, the serum is incubated with measles antigen, which is adhered to a glass microscope slide. Antibodies, if present, will bind to the antigen forming stable antigen-antibody complexes. If no antibodies are present, the complexes will not be formed, and the serum components will be washed away. Fluorescein-labeled antihuman-IgM antibody is added to the reaction side and binds to IgM antibodies if present. This results in a positive reaction of bright apple-green fluorescence when viewed with a fluorescence microscope.(Package insert: Measles Virus Antigen Substrate Slide. AESKU.BION; 09/2019)
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.