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HelpOrder Code LAB5947 Strongyloides Antibody, IgG, Serum
Useful For
Screening for the presence of IgG-class antibodies to Strongyloides
This test is not useful for monitoring patient response to therapy as IgG-class antibodies to Strongyloides may remain detectable following resolution of infection.
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Strongyloides Ab, IgG, SSpecimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat inactivated specimen | Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
The Bordier Strongyloides ratti IgG enzyme-linked immunosorbent assay is an enzymatically amplified sandwich-type immunoassay. After a blocking step, diluted serum and controls are incubated in antigen-coated microtiter wells, then washed and incubated with antihuman-IgG antibody labeled with protein A-alkaline phosphatase conjugate. After a washing step, the wells are incubated with phosphatase substrate. The reaction is halted by potassium phosphate stopping solution and the degree of enzymatic turnover is determined by absorbance measured at 405 nanometers with a reference filter of 590 to 650 nm.(Package insert: Strongyloides Ratti Enzyme immunoassay for the diagnosis of human Strongyloidosis. Bordier Affinity Products SA; 01/2018)
Day(s) Performed
Monday, Wednesday, Thursday, Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682
Testing Algorithm
For more information see Parasitic Investigation of Stool Specimens Algorithm.
Special Instructions
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.